Chronic exposure of human macrophages in vitro to morphine and methadone induces a putative tolerant/dependent state.

نویسندگان

  • Manuel Delgado-Vélez
  • Ana Lugo-Chinchilla
  • Luis Lizardo
  • Irmarie Morales
  • Yarelis Robles
  • Nimaris Bruno
  • José W Rodríguez
  • Eddy Ríos-Olivares
  • Miguel Correa
  • Fernando L Renaud
چکیده

We have shown previously that whereas acute exposure of cultured murine peritoneal macrophages inhibits phagocytosis, chronic exposure results in a putative tolerant/dependent state. We now report similar observations using human cultured monocyte-derived macrophages (hMDM) from a control population and from methadone patients. With hMDM, acute exposure to morphine and methadone inhibited phagocytosis in a dose-dependent manner. In contrast, chronic exposure resulted in eventual normalization of phagocytosis, indicating that a putative tolerant state to the opiates had developed. When opiates were withdrawn from chronically-exposed, tolerized hMDM, phagocytosis was once again depressed. The duration of withdrawal-induced depression lasted several hours, which is much longer than evidenced previously with murine macrophages. These data identify well with various in vivo studies on immune effects of opiate withdrawal; and, in so-doing, supplement ongoing speculation that opiate withdrawal is likely to have serious impact on host defenses of street heroin addicts.

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عنوان ژورنال:
  • Journal of neuroimmunology

دوره 196 1-2  شماره 

صفحات  -

تاریخ انتشار 2008